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Compound five (endophenazine E) featured a phenazine nucleus and also a 6-deoxyhexopyranose sugar as revealed by 1H NMR. Having said that, 7 aromatic protons with overlapped and sophisticated coupling break up designs instructed a monosubstituted as an alternative to a disubstituted pattern.

It's still unclear which genes are accountable for the glycosylation of the endophenazine aglycones, as no this kind of genes have been identified inside or in shut proximity from the epa gene cluster (Table four). Therefore, the genes chargeable for the transfer and methylation on the rhamnose moiety to possibly the carboxyl or the hydroxyl team in the phenazines should be located elsewhere about the chromosome. Investigation to recognize the genes and enzymes liable for the glycosylation in the endaphenazines is at present in development.

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In addition to the glycosylated endophenasides, we were able to clearly show that Kitasatospora sp. MBT66 also makes tiny amounts of a similar endophenazines as produced by Kitasatospora sp. HKI 714 (Supplementary Determine S2 and Supplementary Table S1). We assume that the two strains are carefully associated kitasatosporae, a genus intently connected with Streptomyces within the family of Streptomycetaceae.forty one This could be settled by comparison of your genomes and phenotypic features of the two strains.

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